荧光分光光度法测定海洋生物中的谷胱甘肽     

张静  吉宏武  周静  陈成成  章超桦 《广东海洋大学学报》2005,25(4):32-34

对应用荧光分光光度法测定海洋生物中还原型谷胱甘肽(GSH)含量的方法进行了研究,并测定了分属于鱼、虾、贝、藻的10种海洋生物中谷胱甘肽的含量。利用邻苯二甲醛与GSH反应构成的荧光体系,在激发波长为365nm,发射波长为425nm的条件下,方法的回收率为99.22%~99.69%,变异系数为2.16%。应用此方法测得10种海洋生物中谷胱甘肽的含量为:红笛鲷(Lutjanussanguineus)0.399mg/g,银鲳(Pampusargenteus)0.352mg/g,大海鲢(Megalopscyrinoides)0.561mg/g,尖紫蛤(Sanguinolariaacuta)0.289mg/g,菲律宾蛤仔(Ruditapesphilippinarun)0.287mg/g,墨吉对虾(Penaeusmerguiensis)0.892mg/g,凡纳滨对虾(Litopenaeusvannamei)1.434mg/g,囊藻(Colpomeniasinu-ose)0.221mg/g,石莼(Ulvalactucal)0.727mg/g,马尾藻(Sargassummuticum)0.137mg/g。  相似文献   

夏季台湾浅滩周边水域悬浮物质浊度和叶绿素a荧光分布特征     

胡毅  陈坚  和转 《海洋通报(英文版)》2009,11(2):45-54

2004年7～8月在台湾浅滩周边水域进行了两个断面的水体悬浮物浊度、叶绿素a荧光调查，通过对该区域悬浮物质浊度、叶绿素a荧光的夏季分布变化特征研究，结合水文相关要素分析，初步揭示了区域海洋沉积和再悬浮过程对悬浮体浊度、叶绿素a荧光分布的影响。指出悬浮物质浊度在近岸与陆架外缘主要受再悬浮作用影响，而浅滩区再悬浮作用不明显：在陆架外缘区浮游植物的聚集作用对浊度有一定的贡献作用，而近岸区则不明显。  相似文献   

A method for the quantitative estimation of bacteria from mangrove sediments     

A.H. Dye 《Estuarine, Coastal and Shelf Science》1983,17(2):207-212

A simple and rapid method for the quantitative extraction of bacteria from high silt/clay-content muds associated with mangrove swamps is described. Mud samples are homogenized in filtered sea water and the supernatant is diluted and stained with Acridine Organge. The stained material is trapped on a 0·45 μm membrane filter and bacteria are counted under a fluorescence microscope. Samples may be pre-fixed in 5% formalin if they cannot be examined immediately.  相似文献   

氢化物原子荧光光度法测定地球化学样品中的微量铅     

韦德平 《国土资源》1992,(4)

拟定了用铅空心阴极灯为激发光源,氢化物原子荧光光度法测定地球化学样品中微量铅的方法。在2％HCl-0.08％K_3[Fe(CN)_6]-2％KBH_4体系中,铅的测定灵敏度有明显地提高。该方法操作简便、快速、准确,检出限为8.89×10~(-9)g/ml,精密度为4.12％(n=11)。  相似文献   

A Laser-induced Fluorescence Instrument for Detecting Tropospheric OH and HO2: Characteristics and Calibration   总被引：1，自引：0，他引：1  

Ian C. Faloona  David Tan  Robert L. Lesher  Nathan L. Hazen  Christopher L. Frame  James B. Simpas  Hartwig Harder  Monica Martinez  Piero Di Carlo  Xinrong Ren  William H. Brune 《Journal of Atmospheric Chemistry》2004,47(2):139-167

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Geochemistry of coral from Papua New Guinea as a proxy for ENSO ocean–atmosphere interactions in the Pacific Warm Pool     

Linda K. Ayliffe  Michael I. Bird  Michael K. Gagan  Peter J. Isdale  Heather Scott-Gagan  Bruce Parker  David Griffin  Michael Nongkas  Malcolm T. McCulloch 《Continental Shelf Research》2004,24(19):2343-2356

A Porites sp. coral growing offshore from the Sepik and Ramu Rivers in equatorial northern Papua New Guinea has yielded an accurate 20-year history (1977–1996) of sea surface temperature (SST), river discharge, and wind-induced mixing of the upper water column. Depressions in average SSTs of about 0.5–1.0 °C (indicated by coral Sr/Ca) and markedly diminished freshwater runoff to the coastal ocean (indicated by coral δ¹⁸O, δ¹³C and UV fluorescence) are evident during the El Niño – Southern Oscillation (ENSO) events of 1982–1983, 1987 and 1991-1993. The perturbations recorded by the coral are in good agreement with changes in instrumental SST and river discharge/precipitation records, which are known to be diagnostic of the response of the Pacific Warm Pool ocean–atmosphere system to El Niño. Consideration of coastal ocean dynamics indicates that the establishment of northwest monsoon winds promotes mixing of near-surface waters to greater depths in the first quarter of most years, making the coral record sensitive to changes in the Asian–Australian monsoon cycle. Sudden cooling of SSTs by 1°C following westerly wind episodes, as indicated by the coral Sr/Ca, is consistent with greater mixing in the upper water column at these times. Furthermore, the coral UV fluorescence and oxygen isotope data indicate minimal contribution of river runoff to surface ocean waters at the beginning of most years, during the time of maximum discharge. This abrupt shift in flood-plume behaviour appears to reflect the duration and magnitude of northwest monsoon winds, which tend to disperse flood plume waters to a greater extent in the water column when wind-mixing is enhanced. Our results suggest that a multi-proxy geochemical approach to the production of long coral records should provide comprehensive reconstructions of tropical paleoclimate processes operating on interannual timescales.  相似文献   

光谱波段宽度对森林叶片叶绿素含量反演的影响分析     

佃袁勇  方圣辉  徐永荣  张育育  刘沐阳 《测绘科学》2012,37(6):40-42

随着传感器技术的发展,高光谱数据光谱的波段宽度逐渐变窄,如何从海量的光谱数据中找到最优的光谱波段反演叶绿素含量,成为研究的难点问题。本文在测量华中农业大学狮子山6种主要树种的光谱数据和叶绿素含量的基础上,利用叶绿素指数(CI)和回归方法反演叶绿素含量,并分析了波段宽度对反演叶绿素含量结果的影响,结果发现波段宽度会影响到叶绿素反演的精度,当波段宽度为30nm时,叶绿素含量与"红边"区域(700nm-730nm)和近红外区域(770nm-800nm)叶绿素指标(CI)间的线性关系较好,决定系数可达到77.62%,均方根误差为10.6ug/cm2。  相似文献   

利用窄线宽激光测量锶原子束速率分布     

赵亚楠  吴慧  常宏  张首刚 《时间频率学报》2009,32(1):1-5

利用多普勒频移效应,通过线宽2MHz窄线宽激光与锶原子束在大角度作用下的荧光信号分析,测量了锶原子束速率分布。比较和分析表明,理论结果与实验结果符合较好。  相似文献   

Evidence for a warmer period during the 12th and 13th centuries AD from chironomid assemblages in Southampton Island, Nunavut, Canada     

Nicolas Rolland  Isabelle Larocque  Pierre Francus  Reinhard Pienitz 《Quaternary Research》2009,72(1):27-37

This study presents the Late-Holocene evolution of a northern Southampton Island (Nunavut, Canada) lake, using fossil chironomids supported by sedimentological evidences (XRF, grain size and CNS). All proxies revealed a relatively stable environment during the last millennium with short-lived events driving changes in the entire lake ecosystem. The chironomid-based paleotemperatures revealed variations of significant amplitude coincident with changes in the sediment density and chemical composition of the core. Higher temperature intervals were generally correlated to lower sediment density with higher chironomid concentration and diversity. Higher temperatures were recorded from cal yr AD 1160 to AD 1360, which may correspond to the Medieval Warm Period. Between cal yr AD 1360 and AD 1700, lower temperatures were probably related to a Little Ice Age event. This study presents new information on the timing of known climatic events which will refine our knowledge of the paleoclimate and climatic models of the Foxe Basin region. It also provides a new framework for the evolution of such freshwater ecosystems under the “Anthropocene” and underlines the importance of including sedimentological proxies when interpreting chironomid remains as this combined approach provides an extended overview of the past hydrological and geochemical changes and their impacts on lake biota.  相似文献   

GGB-1型X射线荧光光谱分析高频感应熔样机的性能与应用     

邓赛文  梁国立  刘以建  马天芳 《岩矿测试》2009,28(2):169-172

介绍自行研制的GGB-1型X射线荧光光谱分析高频感应熔样机的特点及性能。采用高频感应加热技术和全自动微机控制技术,设计了温度锁相环控制系统,通过控制高频功率发生器输出振荡电流的大小来控制加热速度和温度,使熔样过程智能化、自动化。研究设计的混匀装置,可同时摇摆与自旋,熔融体形成涡流,使熔融体更均匀。根据X射线荧光光谱分析制样的特点,设计了预氧化过程,更适合于矿石类样品的熔融制样。产品的性能测试结果表明,该熔样机的最高温度为1300℃,温控精度为±2℃,各元素制样精密度优于0.3%(RSD,n=11)。  相似文献